Maria (Cristina) Staiano
Direct: +39 0825 299423
The Laboratory for Molecular Sensing
The main interest of the laboratory is on understanding the rules underlying the folding of proteins and how their structure is modified by the interaction with other proteins, nucleic acids and small ligand molecules. To approach these issues we use, and occasionally develop, new, advanced optical methodologies. The acquired knowledge in protein structure is used for the design of innovative diagnostic methods and for other biotechnology applications.
The focus is to develop and apply advanced luminescence methodologies and optical measurements to solve questions of biochemical and medical interests. In particular, our interests are focused on the study at a molecular level of the interactions involving proteins, enzymes, nuclei acids as well as small ligand molecules for contributing to shed light to the fields of proteomics, post-proteomics, interatomic and bio/sensing. Our lab is equipped with the state-of-art instrumentations for investigating biological interactions by means of FRET, time-resolved FRET, fluorescence polarization, anisotropy decays, fluorescence correlation spectroscopy for single molecule detection, surface plasmon resonance, circular dichroism in the far- and near-UV regions, stopped-flow methodologies, metal enhanced fluorescence. In addition, the Lab has core collaborations in the fields of bioinformatics, atomic force microscopy, linear spectroscopy, material engineering, and nanotechnology that allow us to handle research projects until they are completely fulfilled. The Lab is also active in the use of fluorescence to quantify structural features of biological molecules even at nano-scale, and, consequently, to design advanced optical biochips, Lab-on-Chip, or simple disposable biosensors for analyses of high social interest (POCT). This capability in biophysical methodologies together with expertise in protein biochemistry, molecular genetics and microbiology allows the research team to challenge cutting-edge biochemical questions as well as to rapidly answer to biotechnological requests from SMEs and public institutions.
Keywords: Protein structure and stability; Biosensors; Diagnostic tools; POCT;
Publications (Last five years)
170. A loose domain swapping organization confers a remarkable stability to the dimeric structure of the Arginine Binding Protein from Thermotoga maritima
167. The mKO: An orange-emitting fluorescence protein. Structure and stability
166 A Surface Plasmon Resonance based biochip for the detection of Patulin Toxin
165. The trehalose/maltose-binding protein as a sensitive element of a glucose biosensor
162. The Quaternary Structure of the Recombinant Bovine Odorant-Binding Protein is Modulated by Chemical Denaturants
158. Fluorescence correlation spectroscopy and molecular dynamics simulations to study the structural futures of the maltotriose-binding protein from Thermus thermophilus
155. Amino acid transport in thermophiles: characterization of an arginine-binding protein from Thermotoga maritima. 4. A brief thermo-story
153. Amino acid transport in thermophiles: characterization of an arginine-binding protein from Thermotoga maritima. 3. Conformational dynamics and stability
152. An Innovative Plastic Optical Fiber-based Biosensor for new Bio/applications. The Case of Celiac Disease
151. Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins
150. Under pressure that splits a family in two. The case of lipocalin family
147. Determination of benzyl methyl ketone, a commonly used precursor in amphetamine manufacture
145. Engineering resonance energy transfer for advanced immunoassays: The case of celiac disease
144. Fluorescence-Baserd Biosensors
143. Porous silicon wafer-based “lab on chip” sensor
142. A surface plasmon resonance-based biochip to reveal traces of ephedrine
141. D-Serine-Dehydratase from Saccaromyces cerevisiae. A Pyridoxal -5’-phosphate-Dependent Enzyme for Advanced Biotech Applications
139. New insight in protein-ligand interactions. 2. Stability and properties of two mutant forms of the D-galactose/D-glucose-binding protein from E. coli.
137. Crystallization and preliminary X-ray crystallographic analysis of ligand-free and arginine-bound forms of Thermotoga maritima arginine-binding protein.
136. New insight into protein-ligand interactions. The case of the D-galactose/D-glucose-binding protein from E. coli.
134. Absorption into fluorescence. A method to sense biologically relevant gas molecules
132. Denaturation of Proteins with beta-barrel topology induced by guanidinium hydrochloride.
131.High stability of trehalose/maltose binding protein from Thermococcus litoralis makes it a good candidate as a sensitive element in biosensor systems for sugar control.
130. Structure and stability of D-galactose/D-glucose-binding protein. The role of D-glucose binding and Ca ion depletion
128. Amino acid transport in thermophiles: characterization of an arginine-binding protein in Thermotoga maritima. 2. Molecular organization and structural stability
127. New trends in bio/nanotechnology. Stable proteins as advanced molecular tools for health and environment
126. Crystal structure of an S-formylglutathione hydrolase from Pseudoalteronomas haloplanktis TAC 125
122. Nanostructured silver-based surfaces: new emergent methodologies for an easy detection of analytes
121. Pressure Effects on the structure and stability and of the hyperthermophilic trehalose/maltose-binding protein from Thermococcus litoralis
119. FCS-based sensing for the detection of Ocratoxin A and Neomycin in food
117. Structure and stability of a rat odorant-binding protein. Another brick in the wall”
Patrizia Falabella – Associate Professor University of Basilicata
Paolo Ciambelli – Full Professor University of Salerno
Loredana Incarnato – Full Professor University of Salerno
Emiliano Descrovi – Associate Professor University of Turin
Dr. Marcella de Champdorè
Prof. Petr Herman