Research activity of Beatrice De Giulio is focused on the development and application of advanced analytical methodologies based on mass spectrometry in Food Science and Human Health areas. In particular her studies concern:
I) development and application of molecular profiling strategies based on MALDI-TOF mass spectrometry for food authentication, II) structural characterization of peptides and proteins by mass spectrometry, III) analysis of Volatile Organic Compounds (VOCs) by Solid Phase Micro-Extraction (SPME) coupled to GC-MS to investigate problems of food and clinical interest.
As molecular profiling strategies, rapid and reliable methods to define biomarkers of food authenticity by MALDI-TOF MS analyses have been applied in seafood analysis, in particular to species authentication, verification of commercial product authenticity and detection of fraudulent substitutions.
Structural analyses of peptides and proteins by mass spectrometry have been focused on characterization of tryptic peptides from bovine lactoferrin involved in the inhibition of herpes infection and analysis of haemoglobin peptides modified by epichlorohydrin and diepoxybutane, and are currently targeted to define modifications induced in gliadin peptides by site-specific deamidation and transamidation reactions catalyzed by tissue and/or microbial transglutaminase analyses by nano-HPLC–ESI-MS/MS.
Recently her research activity concerns also SPME GC-MS applications in food flavor analysis. These studies are focused on analysis volatile metabolites to obtain their “profile” in food or on target analysis of compounds that are either restricted, or known as important for quality, processing and storage.
SPME GC-MS strategies in clinical analysis are focused on study the VOCs profiles obtained from urine samples in order to evaluate the potential of the volatile metabolites in the diagnosing and treating of different diseases.
- Cozzolino R., Ramezani S., Martignetti A., Mari A., Piacente S. and De Giulio B. Determination of volatile organic compounds in the dried leaves of Salvia species by solid-phase microextraction coupled to gas chromatography mass spectrometry.
Natural Product Research (Epub ahead of print].
- Cozzolino R., Martignetti A., Pellicano M.P., Stocchero M., Cefola M., Pace B. and De Giulio B.
Characterisation of volatile profile and sensory analysis of fresh-cut “Radicchio di Chioggia” stored in air or modified atmosphere.
Food Chemistry (2015), 192:603- 611.
- Ammendolia M.G., Agamennone M., Pietrantoni A., Lannutti F, Siciliano R.A., De Giulio B., Amici C. and Superti F.
Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.
Pathogens and global health (2012), 106:12-9.
- Cozzolino R. and De Giulio B.
Application of ESI and MALDI-TOF MS for triacylglycerols analysis in edible oils.
European Journal of Lipid Science and Technology (2011), 113: 160-167.
- Mazzeo M.F., De Giulio B., Guerriero G., Ciarcia G., Malorni A., Russo G.L and, Siciliano R.A.,
Fish authentication by MALDI-TOF mass spectrometry.
Journal of Agricultural and Food Chemistry (2008), 56: 11071–11076.
- Terrazzano G., Sica M., Gianfrani C., Mazzarella G., Maurano F., De Giulio B., de Saint-Mezard S., Zanzi D., Maiuri L., Londei M., Jabri B., Troncone R., Auricchio S., Zappacosta S. and Carbone E.
Gliadin regulates the NK-dendritic cell cross talk by HLA-E surface stabilization.
The Journal of Immunology (2007), 179:372-381.
- Salvati V. M., Mazzarella G., Gianfrani C., Levings M.K., Stefanile R., De Giulio B., Iaquinto G., Giardullo N., Auricchio S., Roncarolo M.G. and Troncone R.
Recombinant human IL-10 suppresses gliadin-dependent T-cell activation in ex vivo cultured coeliac intestinal mucosa.
GUT (2005), 54:46-53.
- Massucci M. T., Giansanti F., Di Nino G., Turacchio M., Giardi M. F., Botti D., Ippoliti R., De Giulio B., Siciliano R.A., Donnarumma G., Valenti P., Bocedi A., Polticelli F., Ascenzi P. and Antonini G.
Proteolytic activity of bovine lactoferrin.
BioMetals (2004), 17: 249-255.
- Mazzeo M. F., De Giulio B., Senger S., Rossi M., Malorni A. and Siciliano R.A.
Identification of transglutaminase-mediated deamidation sites in a recombinant α-gliadin by advanced mass-spectrometric methodologies.
Protein Science (2003), 12: 2434-2442.
- Miraglia N., Basile A., Pieri M., Acampora A., Malorni L., De Giulio B. and Sannolo N.
Ion trap mass spectrometry in the structural analysis of haemoglobin peptides modified by epichlorohydrin and diepoxybutane.
Rapid Communication in Mass Spectrometry (2002), 16: 840-847.
- Miraglia N., Pocsfalvi G., Ferranti P., Basile A., Sannolo N., Acampora A., Soleo L., Palmieri F., Caira S., De Giulio B. and Malorni A.
Mass spectrometric identification of a candidate biomarker peptide from the in vitro interaction of epichlorohydrin with red blood cells.
Journal of Mass Spectrometry (2001), 36:47-57.
- Maurano F., Siciliano R. A, De Giulio B., Luongo D., Mazzeo M. F., Troncone R., Auricchio S. and Rossi M.
Intranasal administration of one alpha gliadin can downregulate the immune response to whole gliadin in mice.
Scandinavian Journal of Immunology (2001), 53: 290-295.