Fiorella Mazzeo

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Fiorella Mazzeo

Researcher

Direct: +39 0825 299363
Lab: +39 0825 299151
FAX: +39 0825 781585
Email: fiorella.mazzeo@isa.cnr.it

Short CV

Research Interests

Research activities mainly concern: i) microbial, food and plant proteomics, ii) development and application of molecular profiling strategies based on MALDI-TOF mass spectrometry for food authentication, iii) structural characterization of peptides and proteins by mass spectrometry. Microbial proteomic studies are mainly aimed at identifying proteins, metabolic pathways and molecular processes involved in conferring probiotic features and elucidating stress response mechanisms in technological bacterial species. Plant proteomics is applied to investigate stress response mechanisms and pathogen-host interaction. As molecular profiling strategies, research activity is focused on the development of rapid and reliable methods to define biomarkers of food authenticity by MALDI-TOF MS analyses of protein extracts from fresh and processed food. The method has been applied in seafood analysis, in particular to species authentication, verification of commercial product authenticity and detection of fraudulent substitutions. Similarly, the method was used for European hazelnut cultivars authentication and the potential detection of hazelnut in nut mixtures or in nut containing products. Structural analyses by nano-HPLC–ESI-MS/MS are currently targeted to define modifications induced in gliadin peptides (recognized as celiacogenic epitopes or target of detoxification strategies to reduce wheat flour gluten immunogenicity) by site-specific deamidation and transamidation reactions catalyzed by tissue and/or microbial transglutaminase.

 

Key Publications

  1. Ciarmiello LF, Mazzeo MF, Minasi P, Peluso A, De Luca A, Piccirillo P, Siciliano RA, Carbone V. Analysis of different European hazelnut (Corylus avellana L.) cultivars: authentication, phenotypic features, and phenolic profiles. J Agric Food Chem. 2014; 62:6236-46. PMID: 24927513
  2. Mazzeo MF, Cacace G, Ferriello F, Puopolo G, Zoina A, Ercolano MR, Siciliano RA. Proteomic investigation of response to FORL infection in tomato roots. Plant Physiol Biochem. 2014; 74:42-9. PMID: 24262994
  3. Mazzeo MF, Bonavita R, Maurano F, Bergamo P, Siciliano RA, Rossi M. Biochemical modifications of gliadins induced by microbial transglutaminase on wheat flour. Biochim Biophys Acta. 2013; 1830:5166-74. PMID: 23891939
  4. Lippolis R, Siciliano RA, Mazzeo MF, Abbrescia A, Gnoni A, Sardanelli AM, Papa S. Comparative secretome analysis of four isogenic Bacillus clausii probiotic strains. Proteome Sci. 2013; 11:28. PMID: 23816335
  5. Siciliano RA, Mazzeo MF, Arena S, Renzone G, Andrea Scaloni A. Food Research International 2013; 54: Mass spectrometry for the analysis of protein lactosylation in milk products 988–1000
  6. Mazzeo MF, Cacace G, Peluso A, Zotta T, Muscariello L, Vastano V, Parente E, Siciliano RA Effect of inactivation of ccpA and aerobic growth in Lactobacillus plantarum: A proteomic perspective. J Proteomics. 2012; 75:4050-61. PMID: 22634038
  7. Zotta T, Ricciardi A, Guidone A, Sacco M, Muscariello L, Mazzeo MF, Cacace G, Parente E. Inactivation of ccpA and aeration affect growth, metabolite production and stress tolerance in Lactobacillus plantarum WCFS1. Int J Food Microbiol. 2012; 155:51-9. PMID: 22326142
  8. Siciliano RA, Mazzeo MF. Molecular mechanisms of probiotic action: a proteomic perspective Curr Opin Microbiol. 2012; 15:390-6. PMID: 22538051
  9. Mazzeo MF, Giulio BD, Guerriero G, Ciarcia G, Malorni A, Russo GL, Siciliano RA. Fish authentication by MALDI-TOF mass spectrometry. J. Agric Food Chem. 2008; 56:11071-6. PMID: 19007297
  10. Gianfrani C, Siciliano RA, Facchiano AM, Camarca A, Mazzeo MF,  Costantini S, Salvati VM, Maurano F, Mazzarella G, Iaquinto G, Bergamo P, Rossi M. Gastroenterology. 2007; 133:780-9. PMID: 17678925

 

Research Group

Research Group: Proteomics and Biomolecular Mass Spectrometry Center

Research area: OMICS